Coding

Part:BBa_K3595009:Design

Designed by: Ying Li   Group: iGEM20_GZ_HFI   (2020-10-26)


Mutant cysE-256-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

substituting the ATG (Met) on the 256th position into TGG (Trp) and mutatting the GTC (Val), GAT (Asp) on the 95th and 96th position into AGA (Arg), CCC (Pro).


Source

Synthetic

References